SVF Preclinical Studies

“Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue adipose vs. other adult stem cells” 

SOURCE: Aronowitz JA, Lockhart RA, Hakakian CS. Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue. SpringerPlus 2015;4:713.

Summary: The breakdown of the extracellular matrix achieved with proteolytic enzymes affords significantly greater efficiency to the separation process. SVF isolated through mechanical methods is equally safe, less costly and less time consuming; but, the product contains a higher frequency of blood mononuclear cells and fewer progenitor cells.

“Comparison of multi-lineage cells from human adipose tissue and bone marrow”

SOURCE: De Ugarte DA, Morizono K, Elbarbary A, et al. Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells, tissues, organs 2003;174:101-9.

Summary: The expression of adhesion molecules with known functions in regulating the homing or mobilization of stem cells differs between these 2 cell preparations. ADSCs express CD48d (4-integrin, which forms a heterodimer with CD29 to create very late activation antigen-4, VLA-4, the main cognate ligand for vascular cell adhesion molecule-1 [VCAM-1]–CD106 ligand), whereas cells derived from the bone marrow do not. Similarly, ADSCs express high levels of CD54 (intercellular adhesion molecule-1 [ICAM-1]), whereas BMMSCs have very low expression of this molecule, an important observation, given the important role of the interaction between these adhesion molecules and their ligands in regulating stem cell trafficking.

“Adipose tissue-derived multipotent stromal cells have a higher immunomodulatory capacity than their bone marrow-derived counterparts”

SOURCE: Melief SM, Zwaginga JJ, Fibbe WE, Roelofs H. Adipose tissue-derived multipotent stromal cells have a higher immunomodulatory capacity than their bone marrow-derived counterparts. Stem cells translational medicine 2013;2:455-63.

Summary: Differences in cytokine secretion cause adipose-derived SVF to have more potent immunomodulatory effects than bone marrow-derived MSCs. Therefore, lower numbers of adipose-derived SVF evoke the same level of immunomodulation. The expression of adhesion molecules with known functions in regulating the homing or mobilization of stem cells differs between these 2 cell preparations.

Autologous vs. Allogeneic

“Concise Review: A Safety Assessment of Adipose-Derived Cell Therapy in Clinical Trials: A Systematic Review of Reported Adverse Events” 

SOURCE: Toyserkani NM, Jorgensen MG, Tabatabaeifar S, Jensen CH, Sheikh SP, Sorensen JA.Concise Review: A Safety Assessment of Adipose-Derived Cell Therapy in Clinical Trials: A Systematic Review of Reported Adverse Events. Stem cells translational medicine 2017;6:1786-94.

Summary: The safety of allogeneic SVF has been questioned, especially regarding immunogenicity. It appears that between 19-34% of patients will develop HLA class I antibodies, thereby increasing the risk of subsequent treatments.

Characterization of SVF Cellular Composition

“Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue adipose vs. other adult stem cells” 

SOURCE: Aronowitz JA, Lockhart RA, Hakakian CS. Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue. SpringerPlus 2015;4:713.

Summary: The breakdown of the extracellular matrix achieved with proteolytic enzymes affords significantly greater efficiency to the separation process. SVF isolated through mechanical methods is equally safe, less costly and less time consuming; but, the product contains a higher frequency of blood mononuclear cells and fewer progenitor cells.

Publications using the ICELLATOR® or ADIPASE®

“Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis” 

SOURCE: Doi K, Tanaka S, Iida H, et al. Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis. J Tissue Eng Regen Med 2013;7:864-70.

Summary: The heterogeneous SVF, containing adipose-derived stem/progenitor cells (ADSCs), can be easily isolated through enzymatic digestion of aspirated adipose tissue. In clinical settings, however, strict control of technical procedures according to standard operating procedures and validation of cell-processing conditions are required. Therefore, we evaluated the efficiency and reliability of an automated system for SVF isolation from adipose tissue. SVF cells, freshly isolated using the automated procedure, showed comparable number and viability to those from manual isolation.

“Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review”

SOURCE: van Dongen JA, Tuin AJ, Spiekman M, Jansma J, van der Lei B, Harmsen MC. Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review. J Tissue Eng Regen Med 2017.

Summary: Intraoperative application of the SVF of adipose tissue requires a fast and efficient isolation procedure of adipose tissue. This review was performed to systematically assess and compare procedures currently used for the intraoperative isolation of cellular SVF (cSVF) and tissue SVF (tSVF) that still contain the extracellular matrix. The majority of intraoperative isolation procedures are less time consuming than nonintraoperative control groups, however. Intraoperative isolation procedures are less time-consuming than nonintraoperative control groups with similar cell yield, viability of cells and composition of SVF, and therefore more suitable for use in the clinic. Nevertheless, none of the intraoperative isolation procedures could be designated as the preferred procedure to isolate SVF.

“Point-of-Care Adipose-Derived Stromal Vascular Fraction Cell Isolation and Expanded Polytetrafluoroethylene Graft Sodding”

SOURCE: Stuart K. Williams, PhD, Marvin E. Morris, MD, Paul E. Kosnik, PhD, Kevin D. Lye, MD, Gary D. Gentzkow, MD, Charles B. Ross, MD, Amit J. Dwevidi, MD, and Leigh B. Kleinert. Point-of-Care Adipose-Derived Stromal Vascular Fraction Cell Isolation and Expanded Polytetrafluoroethylene Graft Sodding. TISSUE ENGINEERING: Part C Vol. 23, Number 8, 2017.

Summary: Adipose-derived SVF cell populations are being evaluated for numerous clinical applications. The study evaluated a point-of-care technology, the Tissue Genesis ‘‘TGI 1000’’ Cell Isolation System, to perform an automated isolation of adipose-derived SVF cells to be used in the fabrication of a tissue-engineered vascular graft in the operating room. In conclusion, the ‘‘TGI 1000,’’ provided sufficient cell numbers to perform intra-operating sodding of grafts in the operating room.

For more information on clinical publications related to Tissue Genesis, go to:

https://okyanos.com/cell-therapy-clinical-research/