SVF 임상전 연구

“Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue adipose vs. other adult stem cells” 

출처: Aronowitz JA, Lockhart RA, Hakakian CS. Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue. SpringerPlus 2015;4:713.

요약: 단백질 분해 효소로 달성된 세포외 기질의 파손은 분리 공정에 대한 효율성이 매우 뛰어납니다. SVF isolated through mechanical methods is equally safe, less costly and less time consuming; but, the product contains a higher frequency of blood mononuclear cells and fewer progenitor cells.

“Comparison of multi-lineage cells from human adipose tissue and bone marrow”

출처: De Ugarte DA, Morizono K, Elbarbary A, et al. Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells, tissues, organs 2003;174:101-9.

요약: 줄기 세포의 자동추적 또는 이동을 조절하는 확인 기능으로 접착 분자 발현은 2개의 세포 준비마다 다릅니다. ADSCs express CD48d (4-integrin, which forms a heterodimer with CD29 to create very late activation antigen-4, VLA-4, the main cognate ligand for vascular cell adhesion molecule-1 [VCAM-1]–CD106 ligand), whereas cells derived from the bone marrow do not. Similarly, ADSCs express high levels of CD54 (intercellular adhesion molecule-1 [ICAM-1]), whereas BMMSCs have very low expression of this molecule, an important observation, given the important role of the interaction between these adhesion molecules and their ligands in regulating stem cell trafficking.

“Adipose tissue-derived multipotent stromal cells have a higher immunomodulatory capacity than their bone marrow-derived counterparts”

출처: Melief SM, Zwaginga JJ, Fibbe WE, Roelofs H. Adipose tissue-derived multipotent stromal cells have a higher immunomodulatory capacity than their bone marrow-derived counterparts. Stem cells translational medicine 2013;2:455-63.

요약: 시토카인 분비의 차이는 지방 유래 SVF가 골수 유래 MSC보다 강력한 면역기능 통제 기능을 가지고 있게 합니다. 따라서, 적응 수의 지방 유래 SVF가 동일한 수준의 면역 조절을 자아냅니다. 줄기 세포의 자동추적 또는 이동을 조절하는 확인 기능으로 접착 분자 발현은 2개의 세포 준비마다 다릅니다.

Autologous vs. Allogeneic

“Concise Review: A Safety Assessment of Adipose-Derived Cell Therapy in Clinical Trials: A Systematic Review of Reported Adverse Events” 

출처: Toyserkani NM, Jorgensen MG, Tabatabaeifar S, Jensen CH, Sheikh SP, Sorensen JA.Concise Review: A Safety Assessment of Adipose-Derived Cell Therapy in Clinical Trials: A Systematic Review of Reported Adverse Events. Stem cells translational medicine 2017;6:1786-94.

요약: 동종 이계 SVF의 안전은 면역 성질에 관련될 때 특히 의심되었습니다. It appears that between 19-34% of patients will develop HLA class I antibodies, thereby increasing the risk of subsequent treatments.

SVF 균체 조정 특성화

“Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue adipose vs. other adult stem cells” 

출처: Aronowitz JA, Lockhart RA, Hakakian CS. Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue. SpringerPlus 2015;4:713.

요약: 단백질 분해 효소로 달성된 세포외 기질의 파손은 분리 공정에 대한 효율성이 매우 뛰어납니다. SVF isolated through mechanical methods is equally safe, less costly and less time consuming; but, the product contains a higher frequency of blood mononuclear cells and fewer progenitor cells.

ICELLATOR® 또는 ADIPASE®를 사용한 출판물

“Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis” 

출처: Doi K, Tanaka S, Iida H, et al. Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis. J Tissue Eng Regen Med 2013;7:864-70.

요약: 지방 유래 세포/전구 세포(ADSC)가 함유된 이기종 SVF는 흡인된 지방 조직의 효소 소화를 통해 쉽게 분리할 수 있습니다. In clinical settings, however, strict control of technical procedures according to standard operating procedures and validation of cell-processing conditions are required. Therefore, we evaluated the efficiency and reliability of an automated system for SVF isolation from adipose tissue. SVF cells, freshly isolated using the automated procedure, showed comparable number and viability to those from manual isolation.

“Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review”

출처: van Dongen JA, Tuin AJ, Spiekman M, Jansma J, van der Lei B, Harmsen MC. Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review. J Tissue Eng Regen Med 2017.

요약: 지방 조직 SVF의 상호 운영 적용은 지방 조직의 빠르고 효율적인 분리 절차를 요구합니다. This review was performed to systematically assess and compare procedures currently used for the intraoperative isolation of cellular SVF (cSVF) and tissue SVF (tSVF) that still contain the extracellular matrix. The majority of intraoperative isolation procedures are less time consuming than nonintraoperative control groups, however. Intraoperative isolation procedures are less time-consuming than nonintraoperative control groups with similar cell yield, viability of cells and composition of SVF, and therefore more suitable for use in the clinic. Nevertheless, none of the intraoperative isolation procedures could be designated as the preferred procedure to isolate SVF.

“Point-of-Care Adipose-Derived Stromal Vascular Fraction Cell Isolation and Expanded Polytetrafluoroethylene Graft Sodding”

출처: Stuart K. Williams, PhD, Marvin E. Morris, MD, Paul E. Kosnik, PhD, Kevin D. Lye, MD, Gary D. Gentzkow, MD, Charles B. Ross, MD, Amit J. Dwevidi, MD, and Leigh B. Kleinert. Point-of-Care Adipose-Derived Stromal Vascular Fraction Cell Isolation and Expanded Polytetrafluoroethylene Graft Sodding. TISSUE ENGINEERING: Part C Vol.23, Number 8, 2017.

요약: 지방 조직 SVF 세포군은 수많은 임상 분야를 위해 평가됩니다. The study evaluated a point-of-care technology, the Tissue Genesis ‘‘TGI 1000’’ Cell Isolation System, to perform an automated isolation of adipose-derived SVF cells to be used in the fabrication of a tissue-engineered vascular graft in the operating room. In conclusion, the ‘‘TGI 1000,’’ provided sufficient cell numbers to perform intra-operating sodding of grafts in the operating room.

Tissue Genesis 관련 임상 출판물에 대한 자세한 정보는 다음으로 이동하십시오.

https://okyanos.com/cell-therapy-clinical-research/